RESEARCH ARTICLE A method for extracting high-quality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense Lulu Liu 1☯, Rongchun Han1,2☯, Nianjun Yu, Wei Zhang1, Lihua Xing1, Dongmei Xie1, Daiyin Peng1* 1 School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China, 2 Anhui Innovative Team from …
Sound energy at frequencies greater than 20 KHz is used in ultrasound-assisted extraction (UAE) to disrupt plant cells and release biocompounds from bioresources (Roselló-Soto et al., 2015 ...
Abstract. This chapter presents an overview of the preparation of extracts from plants using organic solvents, with emphasis on common problems encountered and methods for their reduction or elimination. In addition to generally applicable extraction protocols, methods are suggested for selectively extracting specific classes of plant-derived ...
Methods of Extracting Volatile Oils From Plant Material and the Production of Such Oils in the United States .^^ By A. F. SiEVEES, formerly Mochemist, Division of Tobacco ... which give them their characteristic properties. Some oils possess therapeutic properties and are used in medical practice. Others are useful in several ways, as, for ...
1. Introduction. Mung bean (Vigna radiate (L.)) belongs a leguminous plant which has a cultivation history exceeding more than 2000 years in China.It is consumed as a whole snack, bean sprouts, or bean noodles in Asia. Mung bean is one kind of prevalent food in China because of its biological function, such as detoxifying, cholesterol-lowering, anti-tumor and anti …
Processing of plant sample for extraction of protein is usually divided into three steps, i.e., defatting of sample, extraction and precipitation of protein. In defatting, solvents like petroleum ether, n-hexane and n-pentane are used to remove the compounds that interfere with protein extraction.
The properties of the new generation of fluorocarbon solvents have been applied to the extraction of plant materials. The core of the solvent is 1,1,2,2-tetrafluoroethane, better known as hydrofluorocarbon-134a (HFC-134a).
2.3. Ultrasonic-Assisted Extraction. Ultrasound-assisted extraction (UAE) has been used in diverse applications of food-processing technology to extract bioactive compounds from plant materials [].Ultrasound, with levels greater than 20 kHz, is used to disrupt plant cell walls, which helps improve the solvent's ability to penetrate the cells and obtain a higher extraction …
Phenolics are broadly distributed in the plant kingdom and are the most abundant secondary metabolites of plants. Plant polyphenols have drawn increasing attention due to their potent antioxidant properties and their marked effects in the prevention of various oxidative stress associated diseases such as cancer.
Phytochemicals: Extraction Methods, Basic Structures and Mode of Action as Potential Chemotherapeutic Agents James Hamuel Doughari Department of Microbiology, Sch ool of Pure and Applied Sciences, Federal University of Technology, Yola Nigeria 1. Introduction Medicinal plants have been the mainstay of traditional herbal medicine amongst rural
The extraction mostly is based on a physically transfer from the original plant material into another phase which can be separated by filtration. This can be a liquid or even gas depending on the physical properties of the desired compound and the used method. Most common is for the start to use a mixture of organic solvent as ethanol or acetone.
tion plant consists of an extraction unit, distillation units and other related units such as strippers and settlers. The extraction plant in-cludes critical unit operations such as extraction, distillation and ab-sorption [Cho et al., 1999]. Fig. 1 shows a schematic diagram of the sulfolane extraction plant.
The conventional extraction methods, including maceration, percolation and reflux extraction, Some modern or greener extraction methods such as super critical fluid extraction (SFC), pressurized liquid extraction (PLE) and microwave assisted extraction (MAE),are adopted for extracting desired components from natural products.
There are concerns about using synthetic phenolic antioxidants such as butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA) as food additives because of the reported negative effects on human health. Thus, a …
Plant materials with high content of phenolic compounds and flavonoids were found to possess antioxidant properties, and hence are used to treat age-related diseases such as Alzheimer's disease, Parkinsonism, anxiety, and depression.[2,5] Several methods were used in the extraction of medicinal plants such as maceration, infusion, decoction ...
Extraction of Natural Products from Plants – An Introduction Hans - J ö rg Bart 1.1 Introduction The history of the extraction of natural products dates back to Mesopotamian and Egyptian times, where production of perfumes or pharmaceutically - active oils and waxes was a major business.
Extraction methods for preparation of bioactive plant extracts Authors: Waghmare Deepak Harishchandra 1, Deshmukh Arti Madhukar 2 Savitribai Phule Pune University, Pune 1 Vasantrao Naik Marathwada Agriculture University, Parbhani 2 Extraction is the crucial first step in the analysis of medicinal plants, because it is necessary to extract the desired chemical …
Extraction is the separation of medicinally mixture of many plant metabolites, such as alkaloids, glycosides, phenolics, terpenoids, and flavonoids using selective solvents through standard procedures . The aim of all solvent extraction methods is to separate the soluble plant metabolites, leaving behind the insoluble cellular marc.
SOLVENT EXTRACTION. This method employs food grade solvents like hexane and ethanol to isolate essential oils from plant material. It is best suited for plant materials that yield low amounts of essential oil, that are largely …
Extraction of a purified compound. Serdex's historic expertise in the extraction of purified compounds dates to the 1950s during a Franco-Malagasy collaboration to develop and purify triterpene molecules from the dried leaves of Centella asiatica – known for their pharmacological properties for healing wounds.. The process of extracting, separating and …
Plant extraction is a process that aims to extract certain components present in plants. It is a solid/liquid separation operation: a solid object (the plant) is placed in contact with a fluid (the solvent). The plant components of interest are …
Extraction, purification, structural character and biological properties of propolis flavonoids: A review. ... The plant sources of propolis are mainly consisted with plant exudates from bark, buds and etc. Flavonoids are secondary metabolites widely found in natural plants, which have a variety of health care functions and are the main active ...
Plant Medicine: Herbal Extraction Methods. Kristen Myers, lab manager for Turtle Island Herbs in Boulder, Colorado, pours an oil solvent, called a menstruum, onto fresh arnica. The oil and herb will be mixed, then left to soak. …
Plant material. Arabidopsis thaliana seeds (wild-type of ecotype Col-0) were stratified at 4 °C in dark for 3 days before sowing them on soil. The plants were grown in long day (LD) phytotrons that were maintained at 16/8 light/dark …
Major impurities (e.g., from a reaction) should not be soluble in the extracting solvent. Volatility of the extraction solvent. The extracting solvent should be sufficiently volatile so that it can be removed easily from the extracted material by distillation. Toxicity and safety properties of the extraction solvent
Acquiring high quality RNA is the basis of plant molecular biology research, plant genetics and other physiological investigations. At present, a large number of nucleotide isolation methods have been exploited or modified, such as commercial kits, CTAB, SDS methods and so on. Due to the nature of different plants, extraction methods vary. Moreover, efficiency of …
Extraction of Fiber from Sansevieria Trifasciata Plant and its Properties Dr Rita Kant1, Preeti Alagh2 1Assistant Professor, University Institute of Fashion and Lifestyle Technology, Panjab University, Chandigarh 2Assistant Professor, Government Home …
Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties Jin Dai 1, 2 and Russell J. Mumper 3,* 1 Four Tigers LLC, 1501 Bull Lea Road, Suite 105, Lexington, Kentucky 40511 USA; E-Mail: [email protected] (J.D.) 2 Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, Kentucky 40536 ...